The Effect of Fraction and Active Compounds of Momordica Balsamina L. on Bacteria Salmonella Typhi Causing Salmonellosis
Salmonella typhi is one of the bacteria that causes typhus. The handling of typhus by bacteria generally requires the provision of antibacterial substances, namely antibiotics. Excessive and irrational use of antibiotics causes bacteria to be resistant so that over time the benefits of using antibiotics will decrease. Pare leaves are an alternative treatment for various diseases, one of which is antibacterial. The purpose of this study was to determine the ability of bitter melon leaf fractions and active compounds against Salmonella typhi bacteria with various concentrations. This study was a laboratory experimental study through in vitro. The subjects in this research were Salmonella typhi bacteria. The results of this study indicated that the concentration of ethyl acetate fraction is a strong fraction between n-hexane and water methanol fractions in inhibiting Salmonella typhi bacteria. The determination of the active compound group from the purification of the ethyl acetate fraction of forest bitter melon leaves obtained flavonoid active compounds with an Rf value of 0.1 in the eluent n-hexane: ethyl acetate (7:3). The minimum inhibitory concentration (MIC) of ethyl acetate fraction was 125 μg/ml, while the MIC of flavonoid compounds was 62.5 μg/ml against Salmonella typhi bacteria. From the results of the equivalence test of the ethyl acetate fraction with ampicillin against Salmonella typhi bacteria, it showed that the concentration of the active fraction of ethyl acetate 1 μg/ml was equivalent to 0.007 μg/ml ampicillin, while the equivalence of flavonoid compounds was obtained 1 μg/ml concentration of active compounds equivalent to 0.011 μg/ml ampicillin.
Ambarwati. 2007. Effectiveness of Neem Seed Antibacterial Substance (Azadirachta indica) to Inhibit the Growth of Salmonella typhosa and Syaphylococcus aureus. Journal of the Muhammadiyah University of Surakarta. Vol. 8 (3)
Department of health. (2008). National Riskesdas 2007. Agency for Health Research and Development, Ministry of Health, Republic of Indonesia December 2008, 1–384.http://kesga.kemkes.go.id/images/pedoman/Riskesdas 2007 Nasional.pdf
Chambers, SR (2009). No Title 血清 及 尿液 特定 蛋白 检测 在 糖尿病 肾病 早期 诊断 中 的 意义. April.
Handayani, J., Tandelilin. 2006. The Power Influence
Antibacterial Fresh The Leaf Extract (Camelilia Sinensis) Against Streptococcus alpha. Journal of the Indonesian Dentists Association
Holetz, FB (2002). Screening of seme plats used in Brazilian Folk Medizine or the treatment of Infections Disease. Journal of Boline International. 97 (7): 1027-1031
Jawetz, EJL Melnick, EA Adelberg, GF Brooks, JS, Butel, and LNOrnston. 2008. Medical Microbiology. Edition 23. Jakarta
Mahanani, PP, Praharani, D., Purwanto. 2012. Antibacterial Power of Pare Leaf Extract (Momordica Charantia L) In Inhibiting Growth of Streptococcus viridians. Student Research Scientific Articles. University of Jember.
Nurdina, YES (2012). Inhibition of Pare Leaf Extract (Momordica charantia) Against Lactobacillus (Antibacterial Activity of Pare Leaf (Momordica charantia) Extract Against Lactobacillus acidophilus). Scientific Articles of Student Research Results, 3–6.
Oktaviani, Y. 2016. Antibacterial Activity Test of Active Compounds from Pare Leaf Extract (Momordica balsamina L.) Against Escherichia Colidanstaphylococcus Aureus. UniversitasSriwijaya Palembang
Pelczar, M. L and ECS Chan. 2005. Basics of Microbiology (Volume 1). UI Press: Jakarta.
Rahayu, S. (2016). Effect of Concentration of Ethanol Extract of Pare (Momordica charantia L) on the Growth of Shigella dysenteriae Bacteria by In Vitro. Ibn Sina's Scientific Journal, 1 (2), 203–210.
Swartz, S. (2009). STUDY OF ANTIBACTERIAL ACTIVITIES AND IDENTIFICATION OF ANTI-ACTIVE CLASSES OF ANTIBACTERIAL COMPOUNDS OF SIAM (Sechium edule).
Syarifah. 2006. Isolation of Jmabu Bioa Leaf Bacteria Compounds (Eugenia Densilora BL) and Determination of Minimum Inhibitory Concentrations (MIC) against Staphylococcus Aureus and Escherichia coli. Sriwijaya University Postgraduate Thesis Program
Utami, P. 2003. Medicinal Plants to Overcome Diabetes Mellitus. Agro Media Pustaka. Jakarta.
Utami, P. 2012. Natural Antibiotics to Overcome Various Diseases. Agro Media: Jakarta.
Yusran, A. and K. (2009). Anti-fungal inhibition test of Cinnamomun burmanii essential oil extract on the growth of Candida albicans. Dentofacial, 8 (2), 104–110.
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