Rosa Damascena Extract on Angiotensin-Converting Enzyme Activity in Wistar Rats Receiving Sodium Diet

Novita Rachmasari

doi:10.37287/ijghr.v6iS6.5329

Novita Rachmasari Institut Kesehatan Deli Husada Deli Tua

DOI: https://doi.org/10.37287/ijghr.v6iS6.5329

Keywords: ACE, hypertension, rosa damascena

Abstract

The prevalence of hypertension continues to rise in line with lifestyle changes. Often referred to as "the silent killer," hypertension can develop without warning. One of its contributing factors is excessive sodium intake, which disrupts the body's fluid balance regulated by the renin-angiotensin-aldosterone system. Elevated activity of the Angiotensin-Converting Enzyme within the Renin Angiotensin Aldosterone System mechanism can further lead to the development of hypertension. This study aims to investigate the impact of administering ethanol extract of Rosa damascena on the activity of Angiotensin-Converting Enzyme in Wistar rats fed a sodium-enriched diet. This experimental study utilized a post-test-only control group design. The subjects were 30 male Wistar rats, divided into five groups: 1) P0=control group; 2) P1=8% NaCl+RDEE 500mg/kgBW; 3) P2=0.35% NaCl+RDEE 500mg/kgBW; (4) P3=8% NaCl; and (5) P4=0.35% NaCl. The treatments were administered over 28 days. After the treatment period, the rats were anesthetized using a combination of Ketamine and Xylazine. The data were analyzed using a One-Way ANOVA test to assess differences among the treatment groups. A significant difference in Angiotensin-Converting Enzyme activity was observed in Wistar rats before receiving a sodium-enriched diet, with a p-value of <0.05. The ethanol extract of Rosa damascena was proven effective in reducing ACE activity in these rats, with a p-value of <0.05. The ethanol extract of Rosa damascena has demonstrated nephroprotective properties.

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