Perbandingan Gambaran Histologis Lapisan Piramidalis Area Ca1 Hipokampus Mus Musculus yang Dipapar Radiasi Gelombang Elektromagnetik Telepon Seluler 3G dan 4G
Abstract
Penggunaan telepon seluler telah menjadi kebiasaan dan kebutuhan masyarakat modern saat ini. Penggunaan telepon seluler khususnya dalam menelpon sering kali diletakkan pada kepala kanan. Penggunaan ini dapat memberikan dampak kepada hipokampus, salah satunya pada daerah CA1 yang berfungsi untuk membentuk proses ingatan jangka panjang. Tujuan dari penelitian ini adalah untuk mengetahui dampak paparan gelombang elektromagnetik telepon seluler 3G dan 4G pada lapisan piramidalis CA1 hipokampus model hewan coba mencit. Penelitian ini dilakukan secara eksperimental, menggunakan mencit galur Mus musculus jantan, dengan jumlah total sampel sebanyak 32 mencit. Mencit dibagi menjadi 4 kelompok: kelompok kontrol, kelompok Sham, kelompok perlakuan I (P1), dan kelompok perlakuan II (P2). Perlakuan yang diberikan yaitu berupa paparan 3G dan 4G yang dilakukan 1 jam setiap hari selama 30 hari. Variabel yang diteliti adalah ketebalan lapisan dan jumlah neuron pada lapisan piramidal area CA1 hipokampus mencit. Kemudian otak diambil dan dibuat sediaan histologi menggunakan pewarnaan haematoxylin-eosin dan diamati menggunakan mikroskop cahaya pembesaran 400x serta menggunakan software Cell Sens. Analisis data dilakukan dengan uji Anova satu arah dan uji beda nyata terkecil. Hasil penelititan didapatkan bertambahnya ketebalan lapisan piramidal serta berkurangnya rerata jumlah neuron piramidal area CA1 hipokamopus pada kelompok yang diberikan paparan. Setelah dilakukan analisis statistik, tidak terdapat perbedaan bermakna antar kelompok pada ketebalan lapisan piramidalis area CA1 hipokampus serta terdapat perbedaan yang signifikan rerata jumlah neuron piramidal kelompok yang diberikan paparan dan tidak.
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